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1.
Alginate beads containing entrapped DNA were produced using both external and internal calcium sources, and coated with chitosan
or poly-l-lysine membranes. The beads were assayed with DNase nuclease to determine formulation conditions offering the highest level
of DNA protection fromnucleic acid hydrolysis, simulating gastrointestinal exposure. A method was developed to extract and
assay intracapsular DNA through a modified agarose electrophoresis system. Both external and internally gelled beads were
permeable to DNase (Mw=31 kDa), indicated by the absence of DNA after nuclease exposure. At low levels of DNase exposure, coated high guluronic
content alginate beads offered a higher level of DNA protection compared with coated beads with low guluronic alginate. No
apparent correlation was found with chitosan membrane molecular weight and degree of deacetylation; however, increasing poly-l-lysine molecular weight appeared to increase DNase exclusion from beads. At elevated levels of DNase exposure, DNA hydrolysis
was evident within all coated beads with the exception of those coated with the highest molecular weight poly-l-lysine (Mw=197.1 kDa), which provided almost total nuclease protection. Optimal combination then for DNA protection from nucleases is
a high guluronic alginate core, coated with high molecular weight poly-l-lysine. 相似文献
2.
F. P. An et al. 《中国物理 C》2013,37(1):1-20
We report an improved measurement of the neutrino mixing angle θ13 from the Daya Bay Reactor Neutrino Experiment. We exclude a zero value for sin2 θ13 with a significance of 7.7 standard deviations. Electron antineutrinos from six reactors of 2.9 GWth were detected in six antineutrino detectors deployed in two near (flux-weighted baselines of 470 m and 576 m) and one far (1648 m) underground experimental halls. Using 139 days of data, 28909 (205308) electron antineutrino candidates were detected at the far hall (near halls). The ratio of the observed to the expected number of antineutrinos assuming no oscillations at the far hall is 0.944±0.007(stat.)±0.003(syst.). An analysis of the relative rates in six detectors finds sin2 θ13 =0.089±0.010(stat.)±0.005(syst.) in a three-neutrino framework. 相似文献
3.
《Particuology》2018
The evolution of particle size distribution (PSD) of fine polydisperse particles at high number concentrations (>105 cm−3) was simulated through a combined model employing direct quadrature method of moments (DQMOM) with heat and mass transfer equations. The PSD was assumed to retain log-normal distribution during the heterogeneous condensation process. The model was first verified by exact solution and experimental data prior to investigating the influence of initial conditions on final PSD under an octadecane–nitrogen atmosphere. Low particle number concentrations and high vapor concentrations were beneficial to shift the PSD to larger particles having a narrower distribution. Additionally, vapor depletion has more influence on the final PSD than the heat release parameter for a number concentration of 106 cm−3. This study may assist the design process of a gas–solid separating cyclone, to eliminate dust from high-temperature volatiles by pyrolysis of solid fuels. 相似文献
4.
5.
Thin narrow Au stripes suitable for propagating long-range surface plasmon-polaritons were deposited by evaporation and lift-off on a thermal oxide layer on a silicon substrate, and modified by direct adsorption of bovine serum albumin (BSA). Atomic force microscopy (AFM) measurements reveal that BSA adsorbs onto the Au stripes from phosphate buffer solutions forming an adlayer having an average thickness of about 2 nm (surface mass density of about 2 ng/mm2). Comparisons with a simple adsorption model suggest the side-on adsorption of a single monolayer of BSA followed by denaturation and flattening. The BSA-coated stripes have an increased surface roughness compared to a virgin stripe. 相似文献
6.
TOGANO Y AOI N BABA H HONDA T OKADA K HARA Y IEKI K ISHIBASHI Y ITOU Y IWASA N KANNO S KAWABATA T KIMURA H KONDO Y KURITA K KUROKAWA M MORIGUCHI T MURAKAMI H OISHI H OTA S OZAWA A SAKURAI H SHIMOURA S SHIODA R TAKESHITA E TAKEUCHI S YAMADA K YAMADA Y YASUDA Y YONEDA K MOTOBAYASHI T 《中国科学:物理学 力学 天文学(英文版)》2011,(Z1)
Two-proton relative momentum distributions from the break-up channels 23Al→p+p+21Na and 22Mg→p+p+20Ne at an energy of 60-70 A MeV have been measured together with two-proton opening angles at the projectile fragment separator beamline (RIPS) in the RIKEN Ring Cyclotron Facility. The results demonstrate the existence of diproton emission component from single-step 2He for highly excited 23Al and 22Mg. 相似文献
7.
A collection F of operators on a vector space V is said to be
semitransitive if for every pair of nonzero vectors x and y in V
there exists a member T of F such that either Tx = y or Ty = x (or
both). We study semitransitive algebras and semigroups of operators. One
of the main results is that if the underlying field is algebraically
closed, then every semitransitive algebra of operators on a space of dimension n contains a nilpotent element of index n. Among other results on semitransitive semigroups, we show
that if the rank of nonzero members of such a semigroup acting on an
n-dimensional space is a constant k, then k divides n. 相似文献
8.
Combinatorial library screening offers a rapid process for identifying potential therapies to toxins. Hinge peptide libraries,
which rely on conformational diversity rather than traditional molecular diversity, reduce the need for huge numbers of syntheses
and screening steps and greatly expedite the discovery process of active molecules. Hinge peptide libraries having the structures:
Acetyl-X1–X2–hinge–X3–X4–NH2 (capped) and X1–hinge–X2–X3 (uncapped), where X1 through X4 are near-equimolar mixtures of twelve L-amino acids and hinge = 4-aminobutyric acid, were screened for inhibitory activity in bioassays for botulinum neurotoxins
A and B (BoNT/A, BoNT/B) and saxitoxin. The zinc protease activity of the reduced light chains of BoNT/A and /B was assayed
by measuring the cleavage of synthetic substrates. Saxitoxin activity was measured by the restoration of the viability of
neuroblastoma cells treated with ouabain and veratridine. Deconvolution of libraries was accomplished by fixing one position
at a time beginning with the C-terminus. Primary library subsets in which position 4 was fixed showed moderate levels of inhibition
for BoNT/A. Secondary library subsets showed stronger inhibition in the bioassays. In each of the bioassays, inhibitory potency
was stronger when the second position to be fixed was on the opposite side of the hinge, rather than on the same side with
respect to the C-terminus, suggesting that the hinge facilitates the interaction of side chains. Inhibitors for all three
of the toxins studied were discovered within library subsets, although not necessarily in primary subsets. These studies demonstrate
that (1) the best strategy for deconvoluting hinge peptide libraries is by fixing residues alternately on each side of the
hinge moiety, and (2) it is essential to investigate secondary subsets even when primary subsets are inactive. The present
findings support the concept that the increased flexibility imposed by the inclusion of a central hinge residue in small peptides
increases the opportunity for side chain interactions, providing a distinct advantage for hinge peptide libraries over conventional
peptide libraries. Hinge peptide libraries are a rich source of novel ligands for modulation of biomechanisms. The library
subsets uncovered in this study may possess peptides that will lead to effective therapies to neurotoxin poisoning. 相似文献
9.
Numerical Algorithms - This paper is devoted to the derivation of a digital quantum algorithm for the Cauchy problem for symmetric first-order linear hyperbolic systems, thanks to the reservoir... 相似文献
10.
The accurate estimation of outstanding liabilities of an insurance company is an essential task. This is to meet regulatory requirements, but also to achieve efficient internal capital management. Over the recent years, there has been increasing interest in the utilisation of insurance data at a more granular level, and to model claims using stochastic processes. So far, this so-called ‘micro-level reserving’ approach has mainly focused on the Poisson process.In this paper, we propose and apply a Cox process approach to model the arrival process and reporting pattern of insurance claims. This allows for over-dispersion and serial dependency in claim counts, which are typical features in real data. We explicitly consider risk exposure and reporting delays, and show how to use our model to predict the numbers of Incurred-But-Not-Reported (IBNR) claims. The model is calibrated and illustrated using real data from the AUSI data set. 相似文献